During the Symposium of Malta Polyphenols World Congress 2015, Mrs Gras will give a talk about separation for analysis of Anthocyanins by two different methods from different black carrot
Mrs. Gras from the Hohenheim University, will give a talk on June 3 during the symposium on Natural Pigments & Colorants 2015, about Comparison of HPLC and UPLC separation for analysis of anthocyanins from different black carrot varieties (Daucus Carota ssp. Sativus var. Atrorubens Alef).
"Anthocyanins from black carrot (Daucus carota ssp. sativus var. atrorubens Alef.) are natural phenolic pigments widely used for coloring beverages, fruit preparations and confectionary, resulting in a bright red shade at low pH values. They are extraordinarily stable against light and heat exposition due to a high proportion of acylated anthocyanins, which are stabilized via intramolecular co-pigmentation. In the present talk, an ultrasound assisted anthocyanin extraction, their identification and quantitation on a common HPLC-PDA equipped with a C18 core-shell column and modern UPLC-PDA system using a C18 sub-2 µm column were applied and validated. Applying an optimized UPLC separation, differences in the anthocyanin levels in different black carrot varieties will be presented."
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